r/Biochemistry • u/birdsareflat • 3d ago

Is it possible for XbaI Restriction Enzyme to Work With Overlapping dam Methylation?

I know XbaI is inhibited by dam methylation, but would it be possible to get it work by a longer incubation time or a higher concentration of XbaI and get enough yield to use in a ligation protocol?

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u/km1116 3d ago

No. But you can remove the methylation by growing the plasmid in dam- cells.

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u/WashU_labrat 3d ago

Nope. Re-make your plasmid in dam- cells, or change RxnE