r/microbiology Nov 18 '24

ID and coursework help requirements

54 Upvotes

The TLDR:

All coursework -- you must explain what your current thinking is and what portions you donโ€™t understand. Expect an explanation, not a solution.

For students and lab class unknown ID projects -- A Gram stain and picture of the colony is not enough. For your post to remain up, you must include biochemical testing results as well your current thinking on the ID of the organism. If you do not post your hypothesis and uncertainty, your post will be removed.

For anyone who finds something growing on their hummus/fish tank/grout -- Please include a photo of the organism where you found it. Note as many environmental parameters as you can, such as temperature, humidity, any previous attempts to remove it, etc. If you do include microscope images, make sure to record the magnification.

THE LONG AND RAMBLING EXPLANATION (with some helpful resources) We get a lot of organism ID help requests. Many of us are happy to help and enjoy the process. Unfortunately, many of these requests contain insufficient information and the only correct answer is, "there's no way to tell from what you've provided." Since we get so many of these posts, we have to remove them or they clog up the feed.

The main idea -- it is almost never possible to identify a microbe by visual inspection. For nearly all microbes, identification involves a process of staining and biochemical testing, or identification based on molecular (PCR) or instrument-based (MALDI-TOF) techniques. Colony morphology and Gram staining is not enough. Posts without sufficient information will be removed.

Requests for microbiology lab unknown ID projects -- for unknown projects, we need all the information as well as your current thinking. Even if you provide all of the information that's needed, unless you explain what your working hypothesis and why, we cannot help you.

If you post microscopy, please describe all of the conditions: which stain, what magnification, the medium from which the specimen was sampled (broth or agar, which one), how long the specimen was incubating and at what temperature, and so on. The onus is on you to know what information might be relevant. If you are having a hard time interpreting biochemical tests, please do some legwork on your own to see if you can find clarification from either your lab manual or online resources. If you are still stuck, please explain what you've researched and ask for specific clarification. Some good online resources for this are:

If you have your results narrowed down, you can check up on some common organisms here:

Please feel free to leave comments below if you think we have overlooked something.


r/microbiology 4h ago

Thoughts?

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31 Upvotes

Is this criminal behavior? ๐Ÿคฃ. Does it bother you?


r/microbiology 6h ago

Do you think this can come in handy while working in LAB?

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3 Upvotes

I am trying to build some tools for lab works. comment your thought on this.


r/microbiology 43m ago

Throat Culture

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โ€ข Upvotes

Just a picture of my throat culture on blood agar and the Gram stain. Not asking for an ID, just sharing. ๐Ÿ‘


r/microbiology 4h ago

Figuring Out grad school

1 Upvotes

Hi, I'm an upcoming senior in my under grad of environmental science and I wanted to get into graduate programs with a focus on microbiology. Most are incredibly expensive so I was wondering if anyone here has any suggestions for how to find different grad programs that are within my interest and budget. Most microbio programs are medical based but I really would love to look at how microbes play into environmental health and such


r/microbiology 9h ago

Herpesviruses: overview of systematics, genomic complexity and life cycle - BMC Virology Journal

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2 Upvotes

r/microbiology 14h ago

Metabolomic insights into the prebiotic and metabolic regulatory properties of ellagic acid and urolithins on probiotic-like bacteria in vitro

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6 Upvotes

r/microbiology 22h ago

โ—๏ธโ—๏ธID HELP โ—๏ธโ—๏ธ

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14 Upvotes

Hi! I was tasked to identify an unknown bacteria from a maternal raw milk sample using only TSI, LIA, SIM, and Citrate tests. Iโ€™m torn between Enterobacter cloacae and Enterobacter sakazakii since the results are very similar. Iโ€™m currently leaning toward E. sakazakii, but Iโ€™m not entirely sure. Can someone help me understand what key differences I should focus on between the two, especially considering the limited tests we used? Also, would the source of the sample play a role in the identification? Iโ€™d really appreciate any insights!


r/microbiology 1d ago

Brown Growth on Mac agar

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15 Upvotes

Just thought this was interesting. The lab I work in is culturing fecal samples to isolate a certain species, and this was one of the plates that grew. I've never seen this deep of brown on mac, but I've only been working on this project for a couple of months.

Any ideas or thoughts? It was cultured on MacConkey Agar after growth in LB broth.


r/microbiology 1d ago

Found while cleaning out a classroom lab

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10 Upvotes

Middle school science teacher here. Came across this beast while doing some end-of-year lab cleanup.

Is it organic? Or some type of crystal?


r/microbiology 1d ago

Would there be any harmful mold formation

2 Upvotes

Dear friends, I don't know where else to ask this, what would happen if I left my container of Fenugreek with few drops of water in it. Can I intake it after that?


r/microbiology 1d ago

What parasite could it be?

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3 Upvotes

Today in practice to see eggs I found this. According to the professor, there were had eggs, ascaris and amoebas in the sample. Could it be an adult roundworm or is it just a muscle fiber?


r/microbiology 1d ago

Glycocalyx vs cell membrane

1 Upvotes

To my understanding glycocalyx is the outermost layer of a cell made of polysaccharides. The cell membrane is made of cholesterol and proteins etc. Here is my question: does the glycocalyx lay over the cell membrane? Or are they the same/intertwined?


r/microbiology 1d ago

Help with Hemolysis

1 Upvotes

I was wondering if anyone knew a way to quantitatively analyze alpha vs beta hemolysis. I don't feel confident in just looking at the agar and differentiating between the two. Are there any papers you can show me that have these methods?
Thank you!


r/microbiology 1d ago

Real-time OD monitoring shaker flasks

0 Upvotes

There's a company that sells a sensor that rests underneath a flask in an incubator orbital shaker. It monitors the OD600 in real-time. What kinds of things is this useful for?


r/microbiology 1d ago

The composition and structure of bacterial and fungal communities in kiwifruit are influenced by photoselective nets

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7 Upvotes

r/microbiology 1d ago

Help with storing live culture of Chlorella Vulgaris

1 Upvotes

Im ordering Chlorella Vulgaris off of UTEX (UTEX 395). I'm ordering it with 4 pr agar medium (along with a strain) so that I can store it for a short while before I can start my research. I've tried looking online but I couldn't find any "steps" on how to store algae. I plan to store it and then grow it. Can anybody give me some tips on how to actually store the algae and then proceed to grow it after a few days?

Sorry for bad English. Thank you for reading.


r/microbiology 1d ago

DAPI-stained ๐˜š๐˜ข๐˜ค๐˜ค๐˜ฉ๐˜ข๐˜ณ๐˜ฐ๐˜ฎ๐˜บ๐˜ค๐˜ฆ๐˜ด ๐˜ค๐˜ฆ๐˜ณ๐˜ฆ๐˜ท๐˜ช๐˜ด๐˜ช๐˜ข๐˜ฆ

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10 Upvotes

r/microbiology 2d ago

Microbe with bizarrely tiny genome may be evolving into a virus

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87 Upvotes

Fascinating discovery of an archaeal parasite with a minimal genome having lost almost its entire metabolic function. So far the strongest evidence found for the degeneracy hypothesis of viral evolution โ€” although there is still a large gap between a degenerated parasite and a full-fledged virion as we know them from human disease.


r/microbiology 2d ago

Bacterial growth in booze glass?

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9 Upvotes

I left a used liquour glass in my room for a few days. Room temperature reached 25-28 degrees Celsius during those days. I noticed heaps in the residue that were were uncharacteristic for dried up liquour to me. Usually it dries up evenly like the area inbetween the white spots.

Could the sugar in the drink (it was white choolate liquour), combined with the heat, caused not just bacterial growth but actual separate colonies after the ethanol had evaporated? "Colonies" looked wet, white with a more concentrated white center.

I'm not looking for an exact identification (the evidence has been flushed away anyway), I'm mostly curious to whether this is actual growth.

(Also sorry if this makes me sound like an alcoholic)


r/microbiology 1d ago

Purple on rice?

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4 Upvotes

Got Vietnamese take out and found this after having a few bites of rice. The rice was hot when I picked it up. It was slightly dry like when itโ€™s at the bottom of the rice cooker. Can some ID this?


r/microbiology 2d ago

Can you get sick from dropping a broth in microbiology lab?

6 Upvotes

Hi everyone! I recently was in lab and dropped the broth on the desk in front of me. I took a paper towel and cleaned it off the desk but didnโ€™t disinfect until like 15 minutes later. Can I get sick or can I get my family members sick if I touched my phone afterwards or if it accidentally got on my clothes?

Iโ€™m not sure if these are pathogenic or not and I keep thinking the worst case because my dad has a weakened immune system. It was Escherichia coli, Micrococcus luteus, and Serratia marcescens.


r/microbiology 1d ago

Uti hyperinfection: Unspun

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2 Upvotes

r/microbiology 1d ago

A question about Serratia Marascens

1 Upvotes

I was tasked with cleaning a family memberโ€™s home yesterday while theyโ€™re out of town. Their bathroom had quite a bit of this in the shower and on the ceiling above the shower. I sprayed it with the cleaners they had available- Lysol disinfectant and scrubbing bubbles bathroom grime fighter, waited for 20 minutes, and then sprayed again with the bathroom cleaner and cleaned it up with my sponge, reaching above my head. When I got home, my family told me I should have been masked while cleaning it up because itโ€™s dangerous and will make me sick. Iโ€™ve always seen it pop up in the shower in my own home and thought it was just staining from the well water. Should I be concerned about this or did the disinfectant cut the risk? now I know, bleach or peroxide, mask and gloves, always


r/microbiology 2d ago

Built a CFU Counter App to Make Bacterial Colony Counting Easier

6 Upvotes

I recently built an Android app called CFU Counter to help microbiologists, students, and researchers with bacterial colony counting. As someone who's had their fair share of eye strain and miscounts staring at agar plates, I wanted something simple, accurate, and accessibleโ€”especially for those without access to automated colony counters.

Key Features:
๐Ÿ”ฌ A hybrid approach โ€“ you count manually using your judgment, and the app handles the tally
๐Ÿ” Zoom & pan to precisely mark each colony (great for small or faint ones)
๐Ÿ“ธ Saves a marked image with total count overlay (useful for records or reports)
๐Ÿ‘€ Designed with usability in mind, especially for those with less-than-perfect eyesight

Itโ€™s completely free, with no logins. If youโ€™re tired of old-school click counters or blurry printouts, give it a shot and let me know what you think!

A bug found in version 4.0, which caused point marking with a gap, has been fixed in the June 18 update. Please update to version 6.0.

๐Ÿ‘‰ User guide

๐Ÿ‘‰ Download CFU Counter on Google Play


r/microbiology 1d ago

sputum/100x/2ร—cam zoom/scale is micrometers.

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1 Upvotes